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Finally, we highlight the remaining challenges for NADs development, offering references for the design and clinical application of novel NADs
Even in the context of high-fat diet-induced hepatic insulin resistance it has been found the TKT expression in hepatocytes is still sensitive to insulin
2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes
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