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The default TMT-10 workflow was used with the following modifications

Notably the 5 mm length of the spacer pad was such that the gap created between the nitrocellulose membrane and the conjugate pad is sufficiently long to prevent the sampleAuNPanti-B 12 mixture from entering the nitrocellulose membrane at the initial 40 l sample application

This VDF cocktail induced high expression of key hepatic markers such as AFP, HNF4, and Albumin (ALB), with an efficiency of 95.5% (HNF4a+/AFP+ positive cells), closely matching that of the growth factor-based control

Thank you for explaining that there are dietary factors that affect fibroids