Controls received vehicle only, BSO-only group received 125 mg/kg twice daily on days 1, 2 and 3 via intraperitoneal injection, L-PAM-only group received 10 mg/kg dose on days 2 and 3 given intravenously into the lateral tail vein, and the L-PAM+BSO group received both drugs as per above
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The relatively high systemic insulin levels counteract the lipolytic effects of the high GH levels in fat and muscle, but they do not reach adequate levels in the portal vein, so the liver remains under the control of GH, which still induces gluconeogenesis, increasing hyperglycemia [33]
The peptide reaches peak plasma concentrations within 30 minutes and distributes to various tissues including liver, kidney, gastrointestinal tract, and injured tissues