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Drug administration was carried out via intracerebroventricular injection following a standardized method described in previous literature

Anisimov VN, Khavinson VKh, Popovich IG, Zabezhinski MA

The abnormalities of circadian rhythms are related to negative outcomes such as symptom aggravation and recurrence in psychiatric disorders [4, 5]

In contrast, there are studies revealing that the activation of HIF1 signaling is not principally regulated by mutant IDH1, but more likely via other recognized mechanisms like hypoxia, transcriptional regulation, and major genetic and epigenetic alterations induced by gain-of-function or loss-of-function mutations 30