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doi: 10.1369/0022155413497586, 14 BunckM
The deviation from linearity is probably due to geometric constraints in the roGFP barrel similar to those described for roGFP1-iR which had been expected to be less reducing than roGFP1-iL due to stabilization of the thiolate anion by the introduced adjacent basic arginine residue (Lohman and Remington, For live cell measurements biosensors should ideally exhibit a strong preference for one specific analyte
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