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FACS data were analyzed using FlowJo software (version 10.4)

Keep a record of injection dates and sites

The rationale is borrowed from other compounds where tissue saturation and receptor dynamics justify phased dosing

Signs It Should Be Discarded Cloudiness or floating particles Discoloration Expired beyond 28 days post-opening Broken seal or cracked vial Storage Best Practices To ensure bacteriostatic water remains sterile and safe for injection: Store between 20C to 25C (68F to 77F) Avoid direct sunlight or extreme heat Keep the vial sealed when not in use Always use a clean syringe for each draw Bacteriostatic water is effective for up to 28 days after opening, assuming it's stored and handled properly