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Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) was used to examine the enzyme's subunit composition, and its isoelectric points (pI) were determined using chromatofocusing

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Paraxanthine, a less-studied CAF metabolite, has distinct pharmacokinetic and pharmacodynamic properties [6, 12] increasing dopaminergic activity [22] and potentially improving memory consolidation more effectively than caffeine