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Moeller: Yes, fentanyl use has been on a sharp rise over the last several years and is one of the leading causes of opioid overdose deaths
Decrease Stress: Excessive stress raises cortisol levels
Freezing can cause damage due to ice crystal formation, and repeated freeze-thaw cycles will degrade the peptide
Tissue inserts were removed and placed into a 12-well plate containing TEER buffer (1 mL/well, MatTek) and the top surface of each tissue was gently rinsed with 0.5 mL of the same buffer
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