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The selected particles were re-extracted again in Relion with a box size of 256 pixels (bin2, pixel size = 1.692 )

GPX was measured adding cell extract to 50 mmol/l potassium phosphate pH 7.0 containing 1 mM EDTA, 1.5 mM sodium azide, 0.15 IU of purified glutathione reductase from bakers' yeast, 0.45 mM GSH, 0.2 mM NADPH, 1.2 mM cumene hydroperoxide One unit of GPX was defined as the amount of enzyme which produces 1 mol of GSSG (oxidized glutathione)/min at 25C

tomato DC3000 ( Pst DC3000) carrying the avrRpt2 effector (avirulence) gene markedly contributed to the elucidation of plant defense responses in bacterium-infected plants