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Moreover, recent studies have demonstrated a possible beneficial potential of metformin administration for cancer, obesity, non-alcoholic fatty liver disease, polycystic ovary syndrome, metabolic syndrome patients [6] and could even have neuroprotective effects in Alzheimers disease type pathology [7]
PMCID: PMC3845706
PMID: 27021828
Following the final wash, the cell pellet was resuspended in 1.5 ml of PBS containing 0.4% ( v / v ) NP40 alternative and flash-frozen in liquid nitrogen
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