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Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GPx-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated
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It may be more relevant in situations that alter homeostasis (e.g., transport disorders, dialysis, malabsorption) or with very low intakes
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