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(Just keep in mind: RBC testing is not available for all micronutrients, including vitamin D.) What your results actually mean (and why "normal" isn't always optimal) Once your blood is drawn, your levels are compared to a reference range of "normal" values for a nutrient to assess your status
For antibody staining, cells were incubated with Fc block (Human TruStain FcX, BioLegend, 422302, 1:20) for 15 min, then incubated with antibodies in ice-cold 10% FBS, 1 PBS, 2 mM EDTA for 20 min at 4 C and then washed with 1 PBS and resuspended in 10% FBS, 1 PBS and 2 mM EDTA before analysis using a BD LSR Fortessa X-20 flow cytometer with FACS DIVA software (v.9.0.1)
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