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The samples underwent denaturation (10 min at 95C), amplification (40 cycles for 15 s at 95C and 1 min at 60C), quantification using a melting curve program (60 - 99C) with a temperature rate of 0.1C/s, and routine monitoring of fluorescence)
When this enzyme is controlled, excess pigment formation gradually decreases
SafaSMoghaddamGJozaniRJDaghigh KiaHJanmohammadiH
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