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To this end, BALB/c mice, which are genetically deficient in endogenous melatonin production (28), were intranasally challenged with 25 g Alternaria alternata (AA) or PBS daily for four days, with or without concurrent intraperitoneal melatonin treatment using established protocols described previously (2931) ( Figure 1A )

The requirement for baseline lab work receives mixed feedback - some appreciate the thoroughness, others find it a burdensome barrier to getting started