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Obesity is among the most important modifiable risk factors for OSAS
The purified peptide 20GCGRG s Nb35 complex at a concentration of 1214 mg/mL was mixed with 100 M peptide 20 at 4 C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific)
This window is sufficient to observe early changes in body composition and GH axis responsiveness
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