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Carbonyl Content Analysis The carbonyl content was determined by absorbance at 370 nm, and the total carbonyl content was estimated by the difference in absorbance between DNPH-treated and HCl-treated samples, as described by (Reznick and Packer 1994)

While the relatively small sample size, coupled with a notentirely random assignment of groups, may limit generalizability and statistical power in some domains, the convergence of significant, nearsignificant, and biologically relevant trends across independent physiological systems provides strong internal validity and is consistent with prior reports

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