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The outputs from GD_SS1.1 and GD_SS1.2 were pooled and evolved using PANCE on the second stepping-stone, GD_SS2, a substrate containing five additional substitutions from gasdermin D (Fig
Besides, the MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) domain (PF01124) was present only in the TmGst16, indicating that the TmGst16 was a putative membrane bound microsomal Gst protein
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