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Cells were cultured in 200 L Neurobrew (Milteny Biotec, Bergisch Gladbach, Germany), 100 L N2-supplement (Thermo Fisher Scientific, Waltham, MA), 2 L bFGF (PeproTech, Princeton, NJ) and 2 L PDGF-BB (R&D Systems, Minneapolis, MA) per 10 mL of DMEM/F-12 media (Thermo Fisher Scientific, Waltham, MA)

[14] Shimizu, S., Kasai, S., Suzuki, C., Kon, T., Suganuma, H., Suzuki, S., Murashita, K., Nakaji, S., Ihara, K., Tomiyama, M., & Itoh, K