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Rabbit polyclonal antibody against HIF-1 was obtained from (Novus Biologicals, Littleton, CO, USA, #NB100449), while phosphorylated mTOR S2448, -actin, -Tubulin, as well as Glycolysis Antibody Sampler Kit to detect Pfkp, Pkm2, Pkm 1/2, Hexokinase 1, Hexokinase 2 and Pdh were all obtained from (Cell Signaling Technology, Beverly, MA, USA, #2971, #4970, #2148, #8337 respectively)