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Cells were then resuspended with stimulation buffer (Hanks balanced salt solution (HBSS) supplemented with 5 mM HEPES, 0.5 mM IBMX and 0.1% (w/v) BSA, pH 7.4) to a density of 0.6 million cells per mL and added to 384-well white plates (3000 cells per well)
By bypassing the digestive tract, they avoid the first-pass metabolism by the liver
Higher body weight can also reduce the response to some disease-modifying antirheumatic drugs (DMARDs) and biologic medications
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