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Cysteine-mediated redox signaling: chemistry, biology and tools for discovery
"A novel inhibitor of dengue virus replication that targets the capsid protein." Antimicrob Agents Chemother 57(1): 15-25
Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma #IPVH00010), blocked for an hour using 5% milk in TBST, and stained overnight with targeting primary antibodies in 5% milk TBST
Two detection modes were established: fluorescence assay and smartphone-assisted RGB analysis, demonstrating limits of detection (LOD) of 0.12 M and 0.85 M, respectively
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