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Pooled cells in vials were thawed, counted and seeded into wells of six-well plates in RPMI-1640 without phenol red (Gibco, 11835030) supplemented with 10% FBS (Sigma-Aldrich, F7524) at a seeding density of 375,000 cells per well
While protein sources like whey are highly digestible and well absorbed, not all of the amino acids consumed are used directly for protein synthesis in the body
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