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HPLC Method for Analysis of Glutathione reduced (GSH) on Primesep 100 Column June 14, 2023 HPLC Method for Analysis of Glutathione on Primesep 100 by SIELC Technologies Separation type: Liquid Chromatography Mixed-mode L-Glutathione, also known as reduced glutathione or simply GSH, is a tripeptide made up of three amino acids: L-glutamic acid, L-cysteine, and glycine