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The positron emission tomography (PET) was used to examine brain oxygen consumption and cerebral blood flow and revealed that patients with overt hepatic encephalopathy and those with liver cirrhosis without overt hepatic encephalopathy had a lower cerebral metabolic rate of oxygen and cerebral blood flow than healthy subjects 4

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The derivatized samples were transferred to HPLC vials and diluted 5.8 times with 0.25% (v/v) formic acid in 1:1 MeOH: water