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During semaglutide-assisted weight loss, the reduced caloric intake puts muscle mass at risk
The following secondary antibodies were used: Alexa Fluor 555-labelled donkey anti-rabbit IgG (H+L) (#A0453, Beyotime, Shanghai, China, 1:200), horseradish peroxidase (HRP)-labelled goat anti-rabbit IgG (H+L) (#GB23303-1, Servicebio, 1:8000), HRP-labelled goat anti-mouse IgG (H+L) (#GB23301, Servicebio, 1:8000), and HRP-conjugated goat anti-rabbit IgG (#D110,058, Sangon Biotech, 1:8000)
Villi were mechanically dissociated by gentle shaking 3 times and trituration using a 25 mL pipette
These medications require careful dose escalation and ongoing medical monitoring to maximize benefits and minimize side effects
