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Normally, an enzyme called diamine oxidase (DAO) breaks down histamine in the gut, while another enzyme, histamine-N-methyltransferase (HNMT), works inside cells
What separates them is what they were specifically built and validated to do, and that distinction is what determines which one is right for which patient
Add validated solvent slowly down the vial wall, avoid vigorous shaking, and record the final concentration in the laboratory notebook or LIMS
The dilution can reduce the matrix difference between sample and standard to get better accuracy
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