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[9] The critical period appears to be the second trimester

This causes activation of proinflammatory and polyol pathways, increased plasminogen activator inhibitor-1 production, inactivation of important anti-atherosclerotic enzymes (nitric oxide synthase and prostacyclin synthase), raising the level of the atherogenic cholesterol-rich apo B-containing lipoproteins

Next, cells were washed with PBS, incubated for a further 6 to 12 h, lysed, and bacterial enumeration was conducted to assess the internalization capabilities

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