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Results Development and characterization of roGFP2-iL To investigate whether roGFP2 can be modified to generate less reducing probe variants, the roGFP2 sequence was further engineered by introducing site-specific mutations thus generating roGFP2-iL (Figures 1A,B)

LAGB merely constricts gastric volume, whereas SG (resection of ~7080% of the stomach, leaving a tubular remnant) and RYGB (creation of a small gastric pouch anastomosed to the jejunum) fundamentally change gastrointestinal anatomy and physiology

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