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The mRNA expression levels of given genes in the transfected cells with or without cell sorting enrichment were determined by qPCR using Taq388 mix (Du et al., 2022) on a QIAGEN Q-Rex machine (Qiagen, Germany)
Treatment is given by daily subcutaneous injections, traditionally administered at bedtime ( The connections between sleep patterns and growth, learning, attention, and behavioral function are well-established ( In addition, there are physiological reasons to question the efficacy of the evening schedule in improving sleep
Kawakubo Y, Kasai K (2006)
doi: 10.1089/105072503321582060 102 SonHLeeSMYoonRGLeeHLeeIKimSet al
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