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Cocaine neurotoxicity and neurological damage stem from several mechanisms, including mitochondrial dysfunction (Cunha-Oliveira et al., 2010), oxidative stress (Poon et al., 2007), neuroinflammation (Correia et al., 2020), excitotoxicity (Moran et al., 2005), and autophagy (Cao et al., 2017)

For GSH determination 30 minutes after APAP injection, liver tissue (50 mg) was homogenized with 20 volumes of 5% 5-Sulfosalicylic Acid Dihydrate (500 L) (Sigma-Aldrich) and centrifuged at 12,000 g for 10 minutes at 4 C

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This process ensures that the PTT monitoring accurately reflects heparins therapeutic effect, thereby enhancing patient safety and treatment efficacy