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doi: 10.1038/nrendo.2012.140 115 DruckerDJBuseJBTaylorKKendallDMTrautmannMZhuangDet al
Immunofluorescence staining Fibroblasts were fixed, permeabilised, blocked, and incubated overnight at 4 C with primary antibodies targeting p53-pS15 and FOXO4 shown in Table 2, and developed using Alexa Fluor 488-conjugated donkey anti-mouse immunoglobulin G (1:100
Moving into Month 2, I switched to Melanotan 1, injecting twice weekly to maintain my tan
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