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The purity of all proteins was confirmed by analytical SDS-PAGE and the concentrations were determined spectrophotometrically at 280 nm
Benefits of glutathione may include protecting these vital structures from environmental damage and oxidative stress
The intracellular concentration of gamma-glutamylcysteine (GGC) is extremely low (~ 5 uM) when GS is present, thus GCL is considered the rate-limiting enzyme [6]
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