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You'll Lose Water Weight First Within the first 12 weeks of stopping creatine, most people lose 25 pounds this is water that was stored in muscle cells, not fat or muscle tissue

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The adsorbed glycoproteins were eluted at 37 C for 30 min with sample buffer (400 mM Tris-HCl, 10% SDS, 0.5 M 2-mercaptoethanol, 50% glycerol, and 0.025% Bromophenolblau) and resolved on a 10% SDS-polyacrylamide gel

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