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doi:10.3109/00365513.2011.646299, PMID: 22208644 Atkuri KR, Mantovani JJ, Herzenberg LA, Herzenberg LA (2007) N -acetylcysteinea safe antidote for cysteine/glutathione deficiency

The following antibodies were used for immunofluorescence: anti-4HNE (1:200, Abcam, ab46545), anti-GPX4 (1:400, Abcam, ab125066) and anti-GR-1 (1:100, BioLegend, 108413) as primary antibodies and anti-rabbit IgG Alexa Fluor 488 (1:1000, Invitrogen, A-11034) and anti-rat IgG Alexa Fluor 594 (1:1000, Invitrogen, A-11007) as secondary antibody

Mitochondria generate the majority of cellular ROS as a byproduct of oxidative phosphorylation, and mitochondrial GSH is the primary defense against this constant oxidative burden