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Often, Glutathione is called the Master Anti-Oxidant because of its masterful ability to stop, and even reverse, the damage caused by free radicals
Each of these pathways operates on a different timescale and is accelerated by a different environmental factor, which is why a single storage protocol cold, dark, undisturbed, minimal freeze-thaw addresses all three simultaneously rather than requiring separate handling rules for each compound
Gene expression levels were quantified by qPCR using QuantiFast SYBR Green PCR Kit (Qiagen, Chatsworth, CA, USA) and reactions were performed on a Viia TM 7 real time PCR system (Life Technologies, Gaithersburg, MD, USA)