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Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer
avoid freeze-thaw cycles
If you planned 2ml, draw 2ml
Potential pancreatitis risk : As with other GLP-1-based therapies, there is a theoretical concern regarding pancreatitis, though causality has not been firmly established in trials to date
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