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After reaching 60-70 % confluent, they were treated with different conditions respectively
The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates
doi:10.3390/ijms19071987 Pickart L, Vasquez-Soltero J, Margolina A
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