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The electrophoretic mobility shift assay EMSA assays were performed using cell lysates of PAO1 and pqsR mutants as previously described (Gruber et al., pqsA promoter DNA (pqsA), which was generated by PCR using biotinylated forward primer (TTCTTGCTTGGTTGCCG) and reverse primer (GACAGAACGTTCCCTCTT)

However, because patients must wait 30 minutes before eating, this delay may actually help some individuals assess their hunger and nausea levels before consuming food

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