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Soluble extracts were filtered twice through 0.22 m Spin-X column filters (CoStar) and then stored at 80 C until analysis
First pass metabolism typically results in low bioavailability, resulting in adjusted dosages
Use is most often described after age-related collagen decline becomes visible, commonly from the mid-30s onward
In a research context, the main limitations are the gap between animal and human data, stability sensitivity requiring proper storage, and the risk of compromised results if purity is not independently verified
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