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Membranes were blocked with 5% non-fat milk and incubated with mouse anti-5mC (#61480, Active motif) or anti-5hmC (#39999, Active motif) followed by a horseradish peroxidase-conjugated antibody (Santa Cruz Biotechnology)
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We observed that liraglutide treatment led to 224 differentially abundant metabolites in prediabetic mice compared with control mice, 96 metabolites of which showed significantly increased abundances, whereas those of 128 metabolites were decreased
Elshorbagy 2014), dependent on model and experimental setting
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