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GLP-1Ra GLP-1Ra were first marketed for the treatment of T2D (at a lower dose and as daily SC injections), then as weekly injections and finally, at larger doses for the treatment of obesity

Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

Stretching and Mobility Work On injection day, stretching helps improve circulation and reduce soreness or stiffness in the injection area

Rodriguez-Valadez JM, Tahsin M, Fleischmann KE, et al