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Paraffin-embedded brains were sectioned (10 m), blocked with 5% BSA/PBS, and incubated overnight at 4 C with primary antibodies: rat anti-CD16/CD32 (1:200), goat anti-CD206 (1:200), and rabbit anti-Iba1 (1:200)
In addition, proteomic analysis confirmed upregulation of collagen type I and decorin, consistent with dermal remodeling activity
Sheffield-Moore M, Wiktorowicz JE, Soman KV, Danesi CP, Kinsky MP, Dillon EL, Randolph KM, Casperson SL, Gore DC, Horstman AM, Lynch JP, Doucet BM, Mettler JA, Ryder JW, Ploutz-Snyder LL, Hsu JW, Jahoor F, Jennings K, White GR, McCammon SD, Durham WJ
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