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The activity of MC4R is strictly regulated by its endogenous ligands: -melanocyte-stimulating hormone (-MSH) binds to MC4R and transmits anorexigenic and energy-expenditure signals, whereas agouti-related protein (AgRP) exerts an antagonistic effect by binding to the receptor, competitively inhibiting -MSH action and promoting appetite
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The study specifically focused on the liver's ability to produce urea-N (CUNS), which may serve as a measure of the liver's nitrogen processing capabilities
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