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cGAS uniquely binds to DNA and catalyzes the synthesis of cyclic GMPAMP (cGAMP), a second messenger that activates STING, leading to the induction of interferon (IFN) responses via the transcription factor IRF3
(Aspiration remains standard practice for IM injection precisely because muscle is more vascular.) Inject slowly , then withdraw at the same angle and apply gentle pressure with clean gauze
Because each spray delivers a fixed 50mcg + 50mcg dose, researchers can log intake precisely across a study timeline without weighing or measuring liquid volumes
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