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After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h
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optimum level For B12 testing, one of the big problems is that we measure the B12 in blood, but we need it in the cells, and most of all in the brain
It can be transported by PepT1, a transporter that can be upregulated in inflamed gut tissue
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