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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )

Correspondingly, ASBEs were also modulated the expression of STAT3 and caspase 3 proteins and displayed protective role against oxidative and nitrosative stress (426)

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