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After this, the resin was further washed with 25 CVs of buffer containing 20 mM HEPES, pH 7.4, 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl 2 , 1 mM MnCl 2 , 25 M TCEP, 5 M tirzepatide, 0.03% (w/v) LMNG, 0.01% (w/v) glyco-diosgenin (GDN, Anatrace) and 0.008% (w/v) CHS

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Recently, Pyke and colleagues 39 created a mouse anti-GLP-1R monoclonal antibody, which is highly sensitive and specific in the detection of GLP-1R in primate tissues