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For the manufacturing of (2R, 3S)-3-(4-methoxyphenyl) methyl glycidate (a key intermediate for diltiazem) and 3, 4-dihydroxylphenyl alanine (DOPA, for curing of Parkinsons disease) microbial lipases (EC 1.10.3.2) are used [566]

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Western blotting analysis and enzyme-linked immunosorbent assay (ELISA) assay Whole liver lysates were prepared by sonication in HEPES lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 0.1% Tween 20, 10% glycerol, 0.1 mM Na orthovanadate, 1 mM NaF, 10 mM -glycerophosphate) containing protease inhibitor cocktail (EDTA-free Complete Mini, Roche, Meylan, France)