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These transformations showcase real patients who completed physician-supervised weight loss programs at TrimBody M.D
After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)
Protocol source: These schedules reflect practice conventions summarized by a compounding source [18]
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