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Male KKAy mice (10 weeks old) were purchased from Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and maintained in a controlled environment (20 2 C, 5060% humidity) with a 12-h lightdark cycle (lights on at 07:00 and off at 19:00) and free access to water and food
CPT1B activity assay The activity of CPT1B was determined spectrophotometrically by monitoring the release of CoA-SH from palmitoyl-CoA using DTNB [5,5-dithio-bis-(2-nitrobenzoic acid)] (MedChemExpress, HY-15915) as the detection reagent
though they were the best available, we wanted to be able to offer something better
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